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Journal of Clinical Microbiology, November 2007, p. 3529-3533, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.00826-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison between Disk Diffusion and Microdilution Methods for Determining Susceptibility of Clinical Fungal Isolates to Caspofungin

Dipartimento di Igiene e Microbiologia D'Alessandro, Università degli Studi di Palermo, Palermo,¹ Istituto di Malattie Infettive e Medicina, Pubblica Università Politecnica delle Marche, Ancona,² Dipartimento di Scienze Ginecologiche e Microbiologiche, Università degli Studi di Catania, Catania, Italy³

Received 18 April 2007/ Returned for modification 18 May 2007/ Accepted 16 August 2007

We compared the caspofungin (CAS) susceptibility testing results generated by the disk diffusion (DD) assay with the results of the Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BD) reference method for 106 yeast isolates. The isolates represented 11 different fungal species, including Candida albicans (n = 50), C. parapsilosis (n = 10), C. glabrata (n = 10), C. tropicalis (n = 10), C. guillermondii (n = 6), C. rugosa (n = 5), C. krusei (n = 5), C. kefyr (n = 2), C. pelliculosa (n = 2), Saccharomyces cerevisiae (n = 3), and Geotrichum candidum (n = 3). The DD assay was performed in supplemented Mueller-Hinton agar with CAS, which was tested at concentrations of 2, 10, and 25 µg per disk. MICs and inhibition zone diameters were evaluated at 24 and 48 h. In general, the results obtained by the DD assay correlated well with those obtained by the BD method. In particular, a significant correlation between methods was observed when CAS was used at concentration of 2 µg/disk at a reading time of either 24 or 48 h.

Published ahead of print on 29 August 2007.

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