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Journal of Clinical Microbiology, November 2007, p. 3589-3594, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01192-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR{triangledown}

Ingrid Artin,1,2 Per Björkman,3 Jonas Cronqvist,3 Peter Rådström,2 and Elisabet Holst1*

Division of Medical Microbiology, Department of Laboratory Medicine, Lund University, SE-223 62 Lund,1 Applied Microbiology, Lund Institute of Technology, Lund University, SE-221 00 Lund,2 Division of Infectious Diseases, Department of Clinical Sciences, Malmö University Hospital, Lund University, Malmö, Sweden3

Received 13 June 2007/ Returned for modification 4 August 2007/ Accepted 9 September 2007

Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.

* Corresponding author. Mailing address: Division of Medical Microbiology, Department of Laboratory Medicine, Lund University, SE-223 62 Lund, Sweden. Phone: 46-46-17 32 35. Fax: 46-46-18 91 17. E-mail: Elisabet.Holst{at}med.lu.se

{triangledown} Published ahead of print on 19 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3589-3594, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01192-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Artin, I., Carter, A. T., Holst, E., Lovenklev, M., Mason, D. R., Peck, M. W., Radstrom, P. (2008). Effects of Carbon Dioxide on Neurotoxin Gene Expression in Nonproteolytic Clostridium botulinum Type E. Appl. Environ. Microbiol. 74: 2391-2397 [Abstract] [Full Text]  


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