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Genetic Diversity of Paired Middle-Ear and Pharyngeal Nontypeable Haemophilus influenzae Isolates from Children with Acute Otitis Media

Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan,¹ Department of Epidemiology, University of Michigan, Ann Arbor, Michigan,² Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut³

Received 9 May 2007/ Returned for modification 24 July 2007/ Accepted 30 August 2007

Pulsed-field gel electrophoresis was used to determine genetic diversities of multiple nontypeable Haemophilus influenzae isolates from throat and ear specimens of eight children with otitis media. From five children, all ear and throat isolates were identical. The bacterial populations in these specimens showed less diversity than populations in throat isolates of healthy children.

Published ahead of print on 5 September 2007.