This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Amornchai, P.
Right arrow Articles by Peacock, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Amornchai, P.
Right arrow Articles by Peacock, S. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2007, p. 3774-3776, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.00935-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Accuracy of Burkholderia pseudomallei Identification Using the API 20NE System and a Latex Agglutination Test{triangledown}

Premjit Amornchai,1 Wirongrong Chierakul,1 Vanaporn Wuthiekanun,1 Yuvadee Mahakhunkijcharoen,1 Rattanaphone Phetsouvanh,2 Bart J. Currie,3 Paul N. Newton,2,4 Nguyen van Vinh Chau,5 Surasakdi Wongratanacheewin,6 Nicholas P. J. Day,1,4 and Sharon J. Peacock1,4*

Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,1 Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Mahosot Hospital, Vientiane, Lao PDR,2 Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia,3 Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom,4 Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam,5 Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand6

Received 4 May 2007/ Returned for modification 28 June 2007/ Accepted 3 August 2007

In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli.

* Corresponding author. Mailing address: Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. Phone: 66 2 354 1395. Fax: 66 2 354 9169. E-mail: sharon{at}tropmedres.ac

{triangledown} Published ahead of print on 5 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3774-3776, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.00935-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hodgson, K., Engler, C., Govan, B., Ketheesan, N., Norton, R. (2009). Comparison of Routine Bench and Molecular Diagnostic Methods in Identification of Burkholderia pseudomallei. J. Clin. Microbiol. 47: 1578-1580 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»