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Journal of Clinical Microbiology, November 2007, p. 3781-3784, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01603-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Multiple-Locus Variable-Number Tandem-Repeat Analysis for Rapid Typing of Candida glabrata{triangledown}

Frédéric Grenouillet,1,2* Laurence Millon,1,2 Jean-Mathieu Bart,2 Sandrine Roussel,2 Isabelle Biot,1 Emeline Didier,1 Anne-Sophie Ong,1 and Renaud Piarroux1,2

Mycology-Parasitology Department, University Hospital Jean Minjoz,1 SERF (Santé Environnement Rural) Team, EA 2276, Faculty of Medicine, University of Franche-Comté, 25030 Besançon, France2

Received 11 August 2007/ Returned for modification 27 August 2007/ Accepted 31 August 2007

A multiple-locus variable-number tandem-repeat analysis (MLVA) using six microsatellite markers was assessed in 127 Candida glabrata isolates. Thirty-seven different genotypes, stable both in vitro and in vivo, were observed. The highest discriminatory power (D = 0.902) was reached by using only four markers. MLVA seems to be relevant for C. glabrata typing.

* Corresponding author. Mailing address: Mycology-Parasitology Department, CHU Jean Minjoz, 25030 Besançon, France. Phone: (33) 381 66 91 64. Fax: (33) 381 66 8910. E-mail: fgrenouillet{at}chu-besancon.fr

{triangledown} Published ahead of print on 12 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3781-3784, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01603-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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