This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Octavia, S.
Right arrow Articles by Lan, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Octavia, S.
Right arrow Articles by Lan, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2007, p. 3795-3801, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.00720-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Single-Nucleotide-Polymorphism Typing and Genetic Relationships of Salmonella enterica Serovar Typhi Isolates{triangledown} ,{dagger}

Sophie Octavia and Ruiting Lan*

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia

Received 3 April 2007/ Returned for modification 6 August 2007/ Accepted 17 August 2007

Salmonella enterica serovar Typhi is a clone with a low level of variation. We developed a molecular typing method for serovar Typhi using 38 genome-wide single-nucleotide polymorphisms (SNPs) as markers detected by PCR-restriction enzyme digestion. The 73 worldwide serovar Typhi isolates studied were separated into 23 SNP profiles and four distinct genetic groups. Serovar Typhi isolates expressing the unique flagellar antigen z66 were found to cluster together and branch off from the ancestral group, suggesting that serovar Typhi was initially monophasic with only an H1 antigen and subsequently gained the z66 antigen. Typing using the 38 SNPs gave a discriminatory power of 0.87, and a minimum of 16 SNPs may be used to achieve the same level of differentiation. The SNP typing method we developed will be a valuable tool for global epidemiology studies of serovar Typhi.

* Corresponding author. Mailing address: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia. Phone: 61-2 9385 2095. Fax: 61-2 9385 1591. E-mail: r.lan{at}unsw.edu.au

{triangledown} Published ahead of print on 29 August 2007.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, November 2007, p. 3795-3801, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.00720-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Octavia, S., Lan, R. (2009). Multiple-Locus Variable-Number Tandem-Repeat Analysis of Salmonella enterica Serovar Typhi. J. Clin. Microbiol. 47: 2369-2376 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»