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Journal of Clinical Microbiology, November 2007, p. 3802-3803, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01127-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Peptide Nucleic Acid-Fluorescence In Situ Hybridization for Definitive, Rapid Identification of Five Common Candida Species{triangledown}

Megan E. Reller, Amanda B. Mallonee, Nicole P. Kwiatkowski, and William G. Merz*

Division of Medical Microbiology, Department of Pathology, the Johns Hopkins Medical Institutions, Baltimore, Maryland

Received 5 June 2007/ Returned for modification 3 August 2007/ Accepted 27 August 2007

We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.

* Corresponding author. Mailing address: Medical Microbiology Division, Department Pathology, Meyer B1-193, the Johns Hopkins Hospital, 600 N. Wolfe St., Baltimore, MD 21287-7093. Phone: (410) 955-5077. Fax: (410) 614-8087. E-mail: wmerz{at}jhmi.edu

{triangledown} Published ahead of print on 5 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3802-3803, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01127-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trnovsky, J., Merz, W., Della-Latta, P., Wu, F., Arendrup, M. C., Stender, H. (2008). Rapid and Accurate Identification of Candida albicans Isolates by Use of PNA FISHFlow. J. Clin. Microbiol. 46: 1537-1540 [Abstract] [Full Text]  


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