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Journal of Clinical Microbiology, November 2007, p. 3814-3816, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01026-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of "Cronobacter" spp. (Enterobacter sakazakii){triangledown}

Carol Iversen,1,2 Angelika Lehner,1 Niall Mullane,3 John Marugg,2 Séamus Fanning,3 Roger Stephan,1* and Han Joosten2

Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057, Zurich, Switzerland,1 Quality and Safety Department, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland,2 Centre for Food Safety, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland3

Received 17 May 2007/ Returned for modification 29 August 2007/ Accepted 6 September 2007

A taxonomic reclassification of the neonatal pathogen Enterobacter sakazakii to consist of five species within a new genus, "Cronobacter," has recently been proposed. The correct identification of these organisms is important to clinicians. Therefore, using 312 Enterobacteriaceae, including 210 "Cronobacter" strains, the reliabilities of biochemical and genetic confirmation tests were investigated. All "Cronobacter" isolates were positive using dnaG and gluA PCR protocols, and all expressed {alpha}-glucosidase activity. ID32E v3.0 identified 99.5% of "Cronobacter" isolates (as the nearest match to E. sakazakii).

* Corresponding author. Mailing address: Institute for Food Safety and Hygiene, University of Zurich, Winterthurerstrasse 272, CH-8057 Zurich, Switzerland. Phone: 41 44 635 86 51. Fax: 41 44 635 89 80. E-mail: stephanr{at}fsafety.uzh.ch

{triangledown} Published ahead of print on 19 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3814-3816, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01026-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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