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Journal of Clinical Microbiology, November 2007, p. 3821-3823, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01145-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Confirmatory Real-Time PCR Assay for Human Papillomavirus (HPV) Type 52 Infection in Anogenital Specimens Screened for HPV Infection with the Linear Array HPV Genotyping Test

Laboratoire de Virologie Moléculaire, Centre de Recherche, et Départements de Microbiologie et Infectiologie, de Médecine Familiale et Gastro-entérologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada,¹ Département de Microbiologie et Immunologie, Université de Montréal, Montréal, Québec, Canada,² Division of Cancer Epidemiology and Department of Medicine, McGill University, Montreal, Québec, Canada,³ Direction de la Santé Publique de Montréal-Centre, Institut National de Santé Publique du Québec, Montréal, Québec, Canada,⁴ Clinique Médicale l'Actuel, Montréal, Québec, Canada,⁵ Clinique Médicale du Quartier-Latin, Montréal, Québec, Canada,⁶ Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, British Columbia, Canada,⁷ Division of Clinical Investigation and Human Physiology, Toronto General Research Institute, Toronto General Hospital, University of Toronto, Toronto, Ontario, Canada⁸

Received 7 June 2007/ Returned for modification 19 August 2007/ Accepted 15 September 2007

A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.

Published ahead of print on 26 September 2007.

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• Castle, P. E., Gravitt, P. E., Solomon, D., Wheeler, C. M., Schiffman, M. (2008). Comparison of Linear Array and Line Blot Assay for Detection of Human Papillomavirus and Diagnosis of Cervical Precancer and Cancer in the Atypical Squamous Cell of Undetermined Significance and Low-Grade Squamous Intraepithelial Lesion Triage Study. J. Clin. Microbiol. 46: 109-117 [Abstract] [Full Text]