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Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Other PCR-Based Methods for Typing Brucella suis Isolates

Departamento de Microbiología y Parasitología, Universidad de Navarra, 31008 Pamplona, Spain,¹ Université Paris-Sud, Institut de Génétique et Microbiologie, Orsay F-91405, and CNRS, Orsay F-91405, France,² Centre d'Etudes du Bouchet, 5 rue Lavoisier, 91710 Vert le Petit, France,³ Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Gobierno de Aragón, 58080 Zaragoza, Spain,⁴ Croatian Veterinary Institute, Department of Immunology, Savska Cesta 143, 10000, Zagreb, Croatia⁵

Received 31 May 2007/ Returned for modification 31 July 2007/ Accepted 30 September 2007

Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism analysis were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.

Published ahead of print on 17 October 2007.