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Journal of Clinical Microbiology, December 2007, p. 4070-4072, Vol. 45, No. 12
0095-1137/07/$08.00+0     doi:10.1128/JCM.01096-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Other PCR-Based Methods for Typing Brucella suis Isolates{triangledown}

David García-Yoldi,1 Philippe Le Fleche,2,3 María J. De Miguel,4 Pilar M. Muñoz,4 José M. Blasco,4 Zeljko Cvetnic,5 Clara M. Marín,4 Gilles Vergnaud,2,3 and Ignacio López-Goñi1*

Departamento de Microbiología y Parasitología, Universidad de Navarra, 31008 Pamplona, Spain,1 Université Paris-Sud, Institut de Génétique et Microbiologie, Orsay F-91405, and CNRS, Orsay F-91405, France,2 Centre d'Etudes du Bouchet, 5 rue Lavoisier, 91710 Vert le Petit, France,3 Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Gobierno de Aragón, 58080 Zaragoza, Spain,4 Croatian Veterinary Institute, Department of Immunology, Savska Cesta 143, 10000, Zagreb, Croatia5

Received 31 May 2007/ Returned for modification 31 July 2007/ Accepted 30 September 2007

Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism analysis were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.

* Corresponding author. Mailing address: Departamento de Microbiología y Parasitología, Universidad de Navarra, c/ Irunlarrea no. 1, 31008 Pamplona, Spain. Phone: (34) 948 425600. Fax: (34) 948 425649. E-mail: ilgoni{at}unav.es

{triangledown} Published ahead of print on 17 October 2007.

Journal of Clinical Microbiology, December 2007, p. 4070-4072, Vol. 45, No. 12
0095-1137/07/$08.00+0     doi:10.1128/JCM.01096-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kattar, M. M., Jaafar, R. F., Araj, G. F., Le Fleche, P., Matar, G. M., Abi Rached, R., Khalife, S., Vergnaud, G. (2008). Evaluation of a Multilocus Variable-Number Tandem-Repeat Analysis Scheme for Typing Human Brucella Isolates in a Region of Brucellosis Endemicity. J. Clin. Microbiol. 46: 3935-3940 [Abstract] [Full Text]  


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