This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barton, C.
Right arrow Articles by Clark, C. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barton, C.
Right arrow Articles by Clark, C. G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2007, p. 386-391, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01513-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Temperate Bacteriophages Affect Pulsed-Field Gel Electrophoresis Patterns of Campylobacter jejuni{triangledown}

Connie Barton,1 Lai-King Ng,1,2 Shaun D. Tyler,3 and Clifford G. Clark1,2*

Enterics Disease Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St., Winnipeg, MB, Canada R3E 3R2,1 Department of Medical Microbiology, 510 Basic Medical Sciences Building, University of Manitoba, 730 William Avenue, Winnipeg, MB, Canada R3T 2N2,2 DNA Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St., Winnipeg, MB, Canada R3E 3R23

Received 22 July 2006/ Returned for modification 24 September 2006/ Accepted 15 November 2006

The recently sequenced genome of Campylobacter jejuni RM1221 revealed the presence of three integrated bacteriophage-like elements. In this study, genes from the first element, a Mu-like bacteriophage, were amplified by PCR and used to probe pulsed-field gels of clinical C. jejuni strains obtained from a waterborne outbreak (Ontario, Canada, 2000). These highly similar strains differed only by their pulsed-field gel electrophoresis (PFGE) patterns due to an apparent insertion or deletion of a 40-kb fragment. Bacteriophage probes hybridized to these different bands in Southern blot analysis, indicating that homologues of bacteriophage genes were present in the outbreak strains. Investigation of the bacteriophage insertion sites in these isolates suggested that bacteriophage acquisition, loss, or transposition was responsible for the PFGE pattern variation. The bacteriophage gene sequences were similar, but not identical, in the outbreak strains and RM1221, indicating that differences may exist between the bacteriophages.

* Corresponding author. Mailing address: Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, MB, Canada R3E 3R2. Phone: (204) 789-2094. Fax: (204) 789-5012. E-mail: clifford_clark{at}phac-aspc.gc.ca.

{triangledown} Published ahead of print on 29 November 2006.

Journal of Clinical Microbiology, February 2007, p. 386-391, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01513-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sahin, O., Plummer, P. J., Jordan, D. M., Sulaj, K., Pereira, S., Robbe-Austerman, S., Wang, L., Yaeger, M. J., Hoffman, L. J., Zhang, Q. (2008). Emergence of a Tetracycline-Resistant Campylobacter jejuni Clone Associated with Outbreaks of Ovine Abortion in the United States. J. Clin. Microbiol. 46: 1663-1671 [Abstract] [Full Text]  
  • Bielaszewska, M., Prager, R., Kock, R., Mellmann, A., Zhang, W., Tschape, H., Tarr, P. I., Karch, H. (2007). Shiga Toxin Gene Loss and Transfer In Vitro and In Vivo during Enterohemorrhagic Escherichia coli O26 Infection in Humans. Appl. Environ. Microbiol. 73: 3144-3150 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»