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Journal of Clinical Microbiology, February 2007, p. 443-452, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01870-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Using a Resequencing Microarray as a Multiple Respiratory Pathogen Detection Assay{triangledown} ,{dagger}

Baochuan Lin,1* Kate M. Blaney,2 Anthony P. Malanoski,1 Adam G. Ligler,2 Joel M. Schnur,1 David Metzgar,3 Kevin L. Russell,3 and David A. Stenger1

Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375,1 NOVA Research Incorporated, Alexandria, Virginia 22308,2 Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, California 921863

Received 8 September 2006/ Returned for modification 27 October 2006/ Accepted 15 November 2006

Simultaneous testing for detection of infectious pathogens that cause similar symptoms (e.g., acute respiratory infections) is invaluable for patient treatment, outbreak prevention, and efficient use of antibiotic and antiviral agents. In addition, such testing may provide information regarding possible coinfections or induced secondary infections, such as virally induced bacterial infections. Furthermore, in many cases, detection of a pathogen requires more than genus/species-level resolution, since harmful agents (e.g., avian influenza virus) are grouped with other, relatively benign common agents, and for every pathogen, finer resolution is useful to allow tracking of the location and nature of mutations leading to strain variations. In this study, a previously developed resequencing microarray that has been demonstrated to have these capabilities was further developed to provide individual detection sensitivity ranging from 101 to 103 genomic copies for more than 26 respiratory pathogens while still retaining the ability to detect and differentiate between close genetic neighbors. In addition, the study demonstrated that this system allows unambiguous and reproducible sequence-based strain identification of the mixed pathogens. Successful proof-of-concept experiments using clinical specimens show that this approach is potentially very useful for both diagnostics and epidemic surveillance.

* Corresponding author. Mailing address: Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375. Phone: (202) 767-0289. Fax: (202) 767-9594. E-mail: baochuan.lin{at}nrl.navy.mil.

{triangledown} Published ahead of print on 29 November 2006.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, February 2007, p. 443-452, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01870-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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