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Journal of Clinical Microbiology, February 2007, p. 472-476, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.00962-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Pulsed-Field Gel Electrophoresis of Conserved XbaI Fragments for Identification of Swine Salmonella Serotypes{triangledown}

Stephen B. Gaul,1* Stephanie Wedel,1 Matthew M. Erdman,1 D. L. Harris,1,2 Isabel Turney Harris,1 Kathleen E. Ferris,3 and Lorraine Hoffman2

Department of Animal Science,1 Veterinary Diagnostic and Production Animal Medicine, Iowa State University,2 National Veterinary Services Laboratory, Ames, Iowa3

Received 8 May 2006/ Returned for modification 27 August 2006/ Accepted 4 December 2006

Swine Salmonella isolates (n = 674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.

* Corresponding author. Mailing address: Department of Animal Science, Iowa State University, 11 Kildee Hall, Ames, IA 50011.

{triangledown} Published ahead of print on 13 December 2006.

Journal of Clinical Microbiology, February 2007, p. 472-476, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.00962-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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