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Development of a Flow Chart for Identification of Gram-Positive Anaerobic Cocci in the Clinical Laboratory

Research Service,¹ Infectious Diseases Section, Veterans Administration Medical Center West Los Angeles,² Department of Medicine,³ Department of Microbiology, Immunology, and Molecular Genetics, University of California—Los Angeles School of Medicine, Los Angeles, California⁴

Received 8 September 2006/ Returned for modification 24 October 2006/ Accepted 1 November 2006

Gram-positive anaerobic cocci (GPAC) are a heterogeneous group of organisms that are isolated from clinical specimens more often than any group of anaerobic bacteria except Bacteroides species, yet many strains are still difficult or impossible to identify in the diagnostic laboratory. In this study, a total of 124 strains, including 13 reference strains of GPAC species and 111 isolates that had been recovered from clinical specimens previously and identified by 16S rRNA gene sequencing, were subjected to biochemical characterization. Based on the results, a short biochemical scheme that involves the minimum essential biochemical tests for accurate identification of clinically important GPAC was developed.

Published ahead of print on 29 November 2006.

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