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Journal of Clinical Microbiology, February 2007, p. 564-567, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01357-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

PCR-Based Diagnosis of Naegleria sp. Infection in Formalin-Fixed and Paraffin-Embedded Brain Sections{triangledown}

Marc Schild, Christian Gianinazzi, Bruno Gottstein, and Norbert Müller*

Institute of Parasitology, University of Berne, Länggass-Strasse 122, CH-3012 Berne, Switzerland

Received 3 July 2006/ Returned for modification 17 August 2006/ Accepted 15 November 2006

We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline.

* Corresponding author. Mailing address: Institute of Parasitology, Länggass-Strasse 122, CH-3012 Bern, Switzerland. Phone: 41 31 631 24 74. Fax: 41 31 631 26 22. E-mail: nmueller{at}ipa.unibe.ch.

{triangledown} Published ahead of print on 22 November 2006.

Journal of Clinical Microbiology, February 2007, p. 564-567, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01357-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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