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Journal of Clinical Microbiology, February 2007, p. 575-576, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Usefulness of the BACTEC MGIT 960 System for Isolation of Mycobacterium tuberculosis from Sputa Subjected to Long-Term Storage{triangledown}

Manuela Pardini,1 Francis Varaine,2 Maryline Bonnet,2 Graziella Orefici,1 Marco Rinaldo Oggioni,3 the LONG-DRUG Study Group,{dagger} Lanfranco Fattorini,1*

Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy,1 Médecins Sans Frontières, Paris, France,2 Dipartimento di Biologia Molecolare, Università di Siena, Italy3

Received 25 September 2006/ Returned for modification 25 October 2006/ Accepted 7 November 2006

The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 ± 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subjected to long-term storage.

* Corresponding author. Mailing address: Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39 06 49903167. Fax: 39 06 49387112. E-mail: lanfranco.fattorini{at}iss.it.

{triangledown} Published ahead of print on 22 November 2006.

{dagger} The LONG-DRUG study group consists of Marco Rinaldo Oggioni, Francesca Meacci, and Viviana D'Amato, Dipartimento di Biologia Molecolare, Università di Siena, Italy; Graziella Orefici, Lanfranco Fattorini, and Manuela Pardini, Istituto Superiore di Sanità, Rome, Italy; Peter W. Andrew, Mike Barer, and Hasan Yesilkaya, University of Leicester, United Kingdom; Heinz Rinder, LGL, Oberschleißheim, Germany; Sabine Rüsch-Gerdes and Stefan Niemann, Research Centre Borstel, Germany; Germano Orrù, University of Cagliari, Italy; Francis Varaine and Maryline Bonnett, Médecins Sans Frontières, Paris, France; and Thierry Jarosz, 3Es, Paris, France.

Journal of Clinical Microbiology, February 2007, p. 575-576, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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