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Journal of Clinical Microbiology, February 2007, p. 595-599, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01454-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sequencing of the pncA Gene in Members of the Mycobacterium tuberculosis Complex Has Important Diagnostic Applications: Identification of a Species-Specific pncA Mutation in "Mycobacterium canettii" and the Reliable and Rapid Predictor of Pyrazinamide Resistance{triangledown}

Akos Somoskovi,1 Jillian Dormandy,1 Linda M. Parsons,2 Michel Kaswa,1,3 Khye Seng Goh,4 Nalin Rastogi,4 and Max Salfinger1*

Wadsworth Center, New York State Department of Health, Albany, New York,1 International Laboratory Branch, Global AIDS Program, Centers for Disease Control and Prevention, Atlanta, Georgia,2 University of Kinshasa Teaching Hospital, Kinshasa, Democratic Republic of Congo,3 Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Pointe-à-Pitre, Guadeloupe4

Received 13 July 2006/ Returned for modification 19 September 2006/ Accepted 20 November 2006

Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the pncA gene strongly suggests that an alternative mechanism is responsible for PZA resistance. Our results indicate that DNA sequencing of the pncA gene has the potential to shorten the turnaround time and increase the accuracy of PZA susceptibility testing of the M. tuberculosis complex.

* Corresponding author. Present address: Bureau of Laboratories, Florida Department of Health, 4052 Bald Cypress Way, Tallahassee, FL 32399-1716. Phone: (850) 245-4517. E-mail: Max_Salfinger{at}doh.state.fl.us.

{triangledown} Published ahead of print on 29 November 2006.

Journal of Clinical Microbiology, February 2007, p. 595-599, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01454-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Somoskovi, A., Dormandy, J., Mayrer, A. R., Carter, M., Hooper, N., Salfinger, M. (2009). "Mycobacterium canettii" Isolated from a Human Immunodeficiency Virus-Positive Patient: First Case Recognized in the United States. J. Clin. Microbiol. 47: 255-257 [Abstract] [Full Text]  
  • Somoskovi, A., Dormandy, J., Rivenburg, J., Pedrosa, M., McBride, M., Salfinger, M. (2008). Direct Comparison of the GenoType MTBC and Genomic Deletion Assays in Terms of Ability To Distinguish between Members of the Mycobacterium tuberculosis Complex in Clinical Isolates and in Clinical Specimens. J. Clin. Microbiol. 46: 1854-1857 [Abstract] [Full Text]  


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