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Journal of Clinical Microbiology, February 2007, p. 600-603, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01681-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR{triangledown}

Bernd Hoffmann, Timm Harder, Elke Starick, Klaus Depner, Ortrud Werner, and Martin Beer*

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany

Received 15 August 2006/ Returned for modification 9 October 2006/ Accepted 5 December 2006

Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.

* Corresponding author. Mailing address: Friedrich-Loeffler-Institut Insel Riems, Boddenblick 5a, 17493 Greifswald-Insel Riems, D-17493, Germany. Phone: 0049-38351-7-200. Fax: 0049-38351-7-151. E-mail: martin.beer{at}fli.bund.de.

{triangledown} Published ahead of print on 20 December 2006.

Journal of Clinical Microbiology, February 2007, p. 600-603, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01681-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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