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Journal of Clinical Microbiology, February 2007, p. 616-619, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01934-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Improved Multilocus Sequence Typing Scheme for Staphylococcus epidermidis{triangledown}

Jonathan C. Thomas,1 Miguel R. Vargas,1 Maria Miragaia,2 Sharon J. Peacock,3 Gordon L. Archer,4 and Mark C. Enright1*

Department of Infectious Disease Epidemiology, St. Mary's Hospital Campus, Imperial College London, London W2 1PG, United Kingdom,1 Molecular Genetics Laboratory, Instituto de Tecnologia Quimica e Biologica da Universidade Nova de Lisboa (ITQB/UNL), Oeiras, Portugal,2 Wellcome Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,3 Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23298-00494

Received 18 September 2006/ Returned for modification 30 October 2006/ Accepted 27 November 2006

We evaluated three multilocus sequence typing (MLST) schemes for Staphylococcus epidermidis and selected the seven most discriminatory loci for the formation of a new, more powerful MLST scheme. This improved scheme gave 31 sequence types (STs) and 5 clonal complexes (CCs), whereas the other schemes delineate 16 to 24 STs and 1 to 3 CCs.

* Corresponding author. Mailing address: Department of Infectious Disease Epidemiology, St. Mary's Hospital Campus, Imperial College London, London W2 1PG, United Kingdom. Phone: 44 1225 386871. Fax: 44 1225 386779. E-mail: m.c.enright{at}imperial.ac.uk.

{triangledown} Published ahead of print on 6 December 2006.

Journal of Clinical Microbiology, February 2007, p. 616-619, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01934-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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