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Journal of Clinical Microbiology, March 2007, p. 1038-1041, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01103-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Intimin Types Determined by Heteroduplex Mobility Assay of Intimin Gene (eae)-Positive Escherichia coli Strains{triangledown}

Kenitiro Ito,1* Mariko Iida,1 Mitsugu Yamazaki,2 Kazuo Moriya,3 Sanae Moroishi,3 Jun Yatsuyanagi,4 Takayuki Kurazono,5 Noriaki Hiruta,6 and Orn-Anong Ratchtrachenchai7

Infectious Diseases Surveillance Center, National Institute of Infectious Diseases, Tokyo,1 Aichi Prefectural Institute of Public Health, Nagoya,2 Saga Prefectural Center of Public Health and Sanitary Pharmaceutical Research, Saga,3 Akita Prefectural Institute of Public Health, Akita,4 Saitama Institute of Public Health, Saitama,5 Yokosuka Institute of Public Health, Yokosuka, Japan,6 Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand7

Received 30 May 2006/ Returned for modification 3 July 2006/ Accepted 3 January 2007

We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes.

* Corresponding author. Mailing address: Infectious Diseases Surveillance Center, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo 208-0011, Japan. Phone: 81-42561-0771. Fax: 81-42590-8586. E-mail: kito{at}nih.go.jp.

{triangledown} Published ahead of print on 17 January 2007.

Journal of Clinical Microbiology, March 2007, p. 1038-1041, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01103-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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Copyright © 2007 by the American Society for Microbiology. All rights reserved.