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Journal of Clinical Microbiology, March 2007, p. 1053-1057, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02512-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Monitoring of Hepatitis C Virus Quasispecies in Chronic Infection by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Mutation Detection{triangledown}

Carmen Yea,1 Jens Bukh,4 Melissa Ayers,1,{dagger} Eve Roberts,1,2 Mel Krajden,5 and Raymond Tellier1,3*

Metabolism Research Program,1 Division of Gastroenterology,2 Division of Microbiology, The Hospital for Sick Children, Toronto, Canada,3 Hepatitis Viruses Section, NIAID, National Institutes of Health, Bethesda, Maryland; Department of Infectious Diseases, Copenhagen University Hospital, Hvidovre, Denmark,4 British Columbia Centre for Disease Control, Vancouver, Canada5

Received 14 December 2006/ Accepted 4 January 2007

Using both a mass spectrometry-based method and the classical method of cloning and sequencing, we demonstrated weekly changes in the hypervariable region 1 quasispecies of a chimpanzee infected with an infectious clone, coinciding with neutralizing antibody emergence. We also used the mass spectrometry method in the clinical follow-up of a chronically infected patient over a 5-year period.

* Corresponding author. Mailing address: Division of Microbiology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada. Phone: (416) 813-6592. Fax: (416) 813-6257. E-mail: raymond.tellier{at}sickkids.ca.

{triangledown} Published ahead of print on 17 January 2007.

{dagger} Current address: Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.

Journal of Clinical Microbiology, March 2007, p. 1053-1057, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02512-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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