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Journal of Clinical Microbiology, March 2007, p. 725-729, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01572-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of the Capsular Polysaccharides in Staphylococcus aureus Clinical Isolates by PCR and Agglutination Tests{triangledown}

Isabelle Verdier,1 Geraldine Durand,1 Michele Bes,1 Kimberly L. Taylor,2 Gerard Lina,1 François Vandenesch,1 Ali I. Fattom,2 and Jerome Etienne1*

INSERM, E0230, Lyon, F-69008, France, and Université Lyon 1, Centre National de Référence des Staphylocoques, Faculté Laennec, Lyon, F-69008 France,1 W. W. Karakawa Microbial Pathogenesis Laboratory, Nabi Biopharmaceuticals, 12280 Wilkins Avenue, Rockville, Maryland 208522

Received 30 July 2006/ Returned for modification 23 October 2006/ Accepted 17 December 2006

Staphylococcus aureus is a major cause of nosocomial and community-acquired infections. The predominance of two capsular polysaccharides, types 5 and 8, on the surface of clinical isolates led to the development of a conjugate vaccine (StaphVAX) based on capsular polysaccharides types 5 and 8 conjugated to a carrier protein. We have studied the capsular phenotypes and genotypes of 195 isolates representative of all clinical syndromes that encompassed both hospital and community-acquired infections. These isolates were mainly detected in France between January 2001 and December 2004. In this population, most of clinical isolates (87%) expressed either capsular polysaccharide type 5 (42%) or 8 (45%), whereas 13% were nontypeable by the serotyping method with antibodies specific to capsular polysaccharide type 5 or 8. These 26 nontypeable strains were further serotyped and were demonstrated to express the cell wall surface antigen 336, a polyribitol phosphate N-acetylglucosamine, which resembles cell wall teichoic acid. Among methicillin-resistant Staphylococcus aureus (MRSA) strains, we found a predominance of serotype 5 for 64% of strains, whereas MSSA isolates were predominantly capsular serotype 8 (60%). All S. aureus clinical isolates included in the present study have been investigated by PCR method, demonstrating that all isolates carried either the cap5 or the cap8 locus.

* Corresponding author. Mailing address: Centre National de Référence des Staphylocoques, Faculté Laennec, Université Lyon 1, Lyon F-69008, France. Phone: 33478778657. Fax: 33478778658. E-mail: jetienne{at}univ-lyon1.fr.

{triangledown} Published ahead of print on 3 January 2007.

Journal of Clinical Microbiology, March 2007, p. 725-729, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01572-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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