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Journal of Clinical Microbiology, March 2007, p. 730-735, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02317-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Outer Surface Protein Repeat Regions for Improved Genotyping of Staphylococcus epidermidis{triangledown}

Alastair B. Monk and Gordon L. Archer*

Division of Infectious Diseases, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298

Received 15 November 2006/ Returned for modification 11 December 2006/ Accepted 19 December 2006

Staphylococcus epidermidis is an important nosocomial pathogen, but little is known of its epidemiology. Accurate, reproducible typing systems would greatly improve epidemiologic investigations of S. epidermidis. The sequence-based typing technique most recently evaluated, multilocus sequence typing (MLST), often lacks discrimination and can be expensive. PCR and sequence-based analyses of the serine-aspartate repeat region of sdrG (Fbe) and the repeat region of the accumulation-associated protein gene (aap) were evaluated for the ability to discriminate among previously well-characterized S. epidermidis clinical isolates. Forty-eight strains were investigated, with sdrG found in 100% and aap found in 79% of all strains tested. Both genes demonstrated PCR product size and nucleotide sequence variation. Each system by itself gave an index of discrimination similar in value to that of MLST (0.924 and 0.953 compared to 0.96), but discrimination was further improved when combinations of the three systems were used. We conclude that typing systems using amino acid and nucleotide repeat regions of the S. epidermidis surface proteins SdrG and Aap show promise as typing tools and should be investigated using a larger panel of clinically relevant isolates.

* Corresponding author. Mailing address: Virginia Commonwealth University School of Medicine, Sanger Hall, Room 1-018, 1101 East Marshall St., Richmond, VA 23298. Phone: (804) 828-0673. Fax: (804) 828-5022. E-mail: garcher{at}vcu.edu.

{triangledown} Published ahead of print on 3 January 2007.

Journal of Clinical Microbiology, March 2007, p. 730-735, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02317-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McLaws, F., Chopra, I., O'Neill, A. J. (2008). High prevalence of resistance to fusidic acid in clinical isolates of Staphylococcus epidermidis. J Antimicrob Chemother 61: 1040-1043 [Abstract] [Full Text]  


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