This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A retraction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gulati, B. R.
Right arrow Articles by Rao, C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gulati, B. R.
Right arrow Articles by Rao, C. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2007, p. 972-978, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01696-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Diversity in Indian Equine Rotaviruses: Identification of Genotype G10,P6[1] and G1 Strains and a New VP7 Genotype (G16) Strain in Specimens from Diarrheic Foals in India{triangledown}

B. R. Gulati,1,{dagger} R. Deepa,2,{dagger} B. K. Singh,1 and C. Durga Rao2*

National Research Centre on Equines, Sirsa Road, Hisar, Haryana, India,1 Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India2

Received 16 August 2006/ Returned for modification 29 October 2006/ Accepted 17 November 2006

Rotaviruses causing severe diarrhea in foals in two organized farms in northern India, during the period from 2003 to 2005, were characterized by electropherotyping, serotyping, and sequence analysis of the genes encoding the outer capsid proteins. Of 137 specimens, 47 (34.31%) were positive for rotavirus and exhibited at least five different electropherotypes (E), E1 to E5. Strains belonging to different electropherotypes exhibited either a different serotype/genotype specificity or a lack of reactivity to typing monoclonal antibodies (MAbs) used in this study. Strains belonging to E1, E2, and E5 exhibited genotype G10,P6[1], G3, and G1 specificities and accounted for 19.0, 42.9, and 9.5% of the isolates, respectively. Though they possessed G10-type VP7, the E1 strains exhibited high reactivity with the G6-specific MAb, suggesting that the uncommon combination of the outer capsid proteins altered the specificity of the conformation-dependent antigenic epitopes on VP7. E3 and E4 strains accounted for 28.6% of the isolates and were untypeable. Sequence analysis of VP7 from E4 strains (Erv92 and Erv99) revealed that they represent a new VP7 genotype, G16. The detection of unexpected bovine rotavirus-derived G10,P6[1] reassortants, G1 serotype strains, and a new genotype (G16) strain in two distant farms reveals an interesting epidemiological situation and diversity of equine rotaviruses in India.

* Corresponding author. Mailing address: Department of Microbiology & Cell Biology, Indian Institute of Science, Bangalore 560012, India. Phone: 91-80-23602149. Fax: 91-80-23602697. E-mail: cdr{at}mcbl.iisc.ernet.in.

{triangledown} Published ahead of print on 29 November 2006.

{dagger} These authors contributed equally to this work.

Journal of Clinical Microbiology, March 2007, p. 972-978, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01696-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lin, Y.-P., Kao, C.-L., Chang, S.-Y., Taniguchi, K., Hung, P.-Y., Lin, H.-C., Huang, L.-M., Huang, H.-H., Yang, J.-Y., Lee, C.-N. (2008). Determination of Human Rotavirus VP6 Genogroups I and II by Reverse Transcription-PCR. J. Clin. Microbiol. 46: 3330-3337 [Abstract] [Full Text]  
  • Matthijnssens, J., Ciarlet, M., Heiman, E., Arijs, I., Delbeke, T., McDonald, S. M., Palombo, E. A., Iturriza-Gomara, M., Maes, P., Patton, J. T., Rahman, M., Van Ranst, M. (2008). Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains. J. Virol. 82: 3204-3219 [Abstract] [Full Text]  
  • Santos, N., Honma, S., Timenetsky, M. d. C. S. T., Linhares, A. C., Ushijima, H., Armah, G. E., Gentsch, J. R., Hoshino, Y. (2008). Development of a Microtiter Plate Hybridization-Based PCR-Enzyme-Linked Immunosorbent Assay for Identification of Clinically Relevant Human Group A Rotavirus G and P Genotypes. J. Clin. Microbiol. 46: 462-469 [Abstract] [Full Text]  
  • Hong, S.-K., Lee, S.-G., Lee, S.-A, Kang, J.-H., Lee, J.-H., Kim, J.-H., Kim, D.-S., Kim, H.-M., Jang, Y.-T., Ma, S.-H., Kim, S.-Y., Paik, S.-Y. (2007). Characterization of a G11,P[4] Strain of Human Rotavirus Isolated in South Korea. J. Clin. Microbiol. 45: 3759-3761 [Abstract] [Full Text]  
  • Honma, S., Chizhikov, V., Santos, N., Tatsumi, M., do Carmo S. T. Timenetsky, M., Linhares, A. C., Mascarenhas, J. D. P., Ushijima, H., Armah, G. E., Gentsch, J. R., Hoshino, Y. (2007). Development and Validation of DNA Microarray for Genotyping Group A Rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) Genes. J. Clin. Microbiol. 45: 2641-2648 [Abstract] [Full Text]  
  • Phan, T. G., Okitsu, S., Maneekarn, N., Ushijima, H., Duan, Z.-J. (2007). Possible Misidentification of GSP[6] Rotavirus as a Novel Strain Detected in Humans for the First Time. J. Clin. Microbiol. 45: 2098-2099 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»