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Journal of Clinical Microbiology, April 2007, p. 1225-1233, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02199-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Discovery of a New Capsular Serotype (6C) within Serogroup 6 of Streptococcus pneumoniae{triangledown}

In Ho Park,1 David G. Pritchard,2 Rob Cartee,3 Angela Brandao,4,5 Maria Cristina C. Brandileone,5 and Moon H. Nahm1,3*

Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama,1 Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama,2 Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama,3 IOC/FIOCRUZ, Rio de Janeiro, Brazil,4 Pyogenic and Toxigenic Bacteria Laboratory, Bacteriology Department, Adolfo Lutz Institute, São Paulo, Brazil5

Received 26 October 2006/ Returned for modification 10 January 2007/ Accepted 22 January 2007

Using two monoclonal antibodies, we found subtypes among pneumococcal isolates that are typed as serotype 6A by the quellung reaction. The prevalent subtype bound to both monoclonal antibodies and was labeled here 6A{alpha}, whereas the minor subtype bound to only one monoclonal antibody and was labeled 6Aß. To determine the biochemical nature of the two serologically defined subtypes, we purified capsular polysaccharides (PSs) from the two subtypes and examined their chemical structures with gas-liquid chromatography and mass spectrometry. The study results for 6A{alpha} PS are consistent with the previously published structure of 6A PS, which is ->2) galactose (1->3) glucose (1->3) rhamnose (1->3) ribitol (5->phosphate. In contrast, the 6Aß PS study results show that its repeating unit is ->2) glucose 1 (1->3) glucose 2 (1->3) rhamnose (1->3) ribitol (5->phosphate. We propose to continue referring to 6A{alpha} as serotype 6A but to refer to 6Aß as serotype 6C. Serotype 6C would thus represent the 91st pneumococcal serotype, with 90 pneumococcal serotypes having previously been recognized. This study also demonstrates that a new serotype may exist within an established and well-characterized serogroup or serotype.

* Corresponding author. Mailing address: Department of Pathology, University of Alabama at Birmingham, 845 19th Street South (BBRB 614), Birmingham, AL 35294. Phone: (205) 934-0163. Fax: (205) 975-2149. E-mail: nahm{at}uab.edu

{triangledown} Published ahead of print on 31 January 2007.

Journal of Clinical Microbiology, April 2007, p. 1225-1233, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02199-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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