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Journal of Clinical Microbiology, April 2007, p. 1238-1243, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.01188-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of "Haematobacter," a New Genus of Aerobic Gram-Negative Rods Isolated from Clinical Specimens, and Reclassification of Rhodobacter massiliensis as "Haematobacter massiliensis comb. nov."{triangledown}

Leta O. Helsel,1* Dannie Hollis,1 Arnold G. Steigerwalt,1 Roger E. Morey,1 Jean Jordan,1 Tin Aye,1,{dagger} Jon Radosevic,3 Deanna Jannat-Khah,1 Dorothy Thiry,4 David R. Lonsway,2 Jean B. Patel,2 Maryam I. Daneshvar,1 and Paul N. Levett1,4

Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases,1 Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,2 Indiana State Department of Health, Indianapolis, Indiana,3 Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada4

Received 8 June 2006/ Returned for modification 30 August 2006/ Accepted 29 January 2007

Twelve strains of gram-negative, nonfermenting rods recovered mainly from septicemic patients were studied using conventional and molecular methods. The phenotypic profiles of these strains most closely resembled Psychrobacter phenylpyruvicus. They produced catalase, oxidase, urease, and H2S (lead acetate paper) but did not produce indole, reduce nitrate or nitrite, or hydrolyze gelatin or esculin. No acid production was observed in a King's oxidation-fermentation base containing D-glucose, D-xylose, D-mannitol, sucrose, lactose, or maltose. All strains were nonmotile and nonpigmented. Most strains produced green discoloration on blood agar. All strains grew at 25°C and 35°C and most grew on MacConkey agar. They shared a common cellular fatty acid (CFA) profile characterized by large amounts (56% to 90%) of 18:1{omega}7c and the presence of 3-OH-10:0, 16:1{omega}7c, 16:0, and 19:0cyc{omega}8c that overall was most similar to that of Rhodobacter species but was quite distinct from that of P. phenylpyruvicus. The MICs for most ß-lactams, fluoroquinolones, aminoglycosides, and carbapenems were low. MICs for aztreonam and piperacillin were higher, with MICs for some strains of > 64 mg/liter and > 128 mg/liter, respectively. Polyphasic analysis of these strains, including morphological, biochemical, CFA composition, DNA-DNA hybridization, 16S rRNA gene sequencing, and percent guanine-plus-cytosine (G+C) content analysis, demonstrated that these strains and Rhodobacter massiliensis represent a new genus, "Haematobacter" (proposed name), with the species H. missouriensis (type strain H1892T = CCUG 52307T = CIP 109176T) and H. massiliensis comb. nov. (type strain FramboiseT = CCUG 47968T = CIP 107725T) and an unnamed genomospecies.

* Corresponding author. Mailing address: Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-5458. Fax: (404) 639-3022. E-mail: LOH1{at}cdc.gov

{triangledown} Published ahead of print on 7 February 2007.

{dagger} Deceased.

Journal of Clinical Microbiology, April 2007, p. 1238-1243, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.01188-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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