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Journal of Clinical Microbiology, April 2007, p. 1305-1307, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02502-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Real-Time PCR Assay Using Specimens on Filter Disks as a Template for Detection of Cytomegalovirus in Urine{triangledown}

Naoki Nozawa,1 Shin Koyano,2 Yumiko Yamamoto,1 Yuhki Inami,1 Ichiro Kurane,1 and Naoki Inoue1*

Department of Virology I, National Institute of Infectious Diseases, Tokyo,1 Department of Pediatrics, Asahikawa Medical College, Hokkaido, Japan2

Received 14 December 2006/ Returned for modification 24 January 2007/ Accepted 29 January 2007

Since congenital cytomegalovirus (CMV) infection causes late-onset sequelae, the identification of CMV-infected newborns is important. For this purpose, we established a simple real-time PCR assay using a filter disk. Combined with the collection of urine using filter papers placed in the diaper, this assay can make CMV screening more feasible and cost-effective.

* Corresponding author. Mailing address: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111. Fax: 81-3-5285-1180. E-mail: ninoue{at}nih.go.jp

{triangledown} Published ahead of print on 7 February 2007.

Journal of Clinical Microbiology, April 2007, p. 1305-1307, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02502-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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  • Stehel, E. K., Shoup, A. G., Owen, K. E., Jackson, G. L., Sendelbach, D. M., Boney, L. F., Sanchez, P. J. (2008). Newborn Hearing Screening and Detection of Congenital Cytomegalovirus Infection. Pediatrics 121: 970-975 [Abstract] [Full Text]  


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