This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, L. M.
Right arrow Articles by Afonso, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, L. M.
Right arrow Articles by Afonso, C. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2007, p. 1310-1314, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02594-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR{triangledown} ,{dagger}

L. Mia Kim,1 Daniel J. King,1 David L. Suarez,1 Chun W. Wong,2 and Claudio L. Afonso1*

USDA-ARS Southeast Poultry Research Laboratory, 934 College Station Rd., Athens, Georgia 30605,1 Tai Lung Veterinary Laboratory, Agriculture, Fisheries and Conservation Department, Lin Tong Mei, Sheung Shui, NT, Hong Kong, SAR, People's Republic of China2

Received 27 December 2006/ Accepted 16 January 2007

Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.

* Corresponding author. Mailing address: Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Rd., Athens, GA 30605. Phone: (706) 546-3642. Fax: (706) 546-3161. E-mail: cafonso{at}seprl.usda.gov

{triangledown} Published ahead of print on 7 February 2007.

{dagger} Supplemental material for this article may be found at http:/jcm.asm.org.

Journal of Clinical Microbiology, April 2007, p. 1310-1314, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02594-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mia Kim, L., Suarez, D. L., Afonso, C. L. (2008). Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real-time reverse transcription polymerase chain reaction assay. jvdi 20: 414-425 [Abstract] [Full Text]  
  • Choi, K.-S., Lee, E.-K., Jeon, W.-J., Nah, J.-J., Kim, Y.-J., Lee, M.-Y., Lee, H., Kwon, J.-H. (2008). Isolation of a Recent Korean Epizootic Strain of Newcastle Disease Virus from Eurasian Scops Owls Affected with Severe Diarrhea. J Wildl Dis 44: 193-198 [Abstract] [Full Text]  
  • Kim, L. M., King, D. J., Curry, P. E., Suarez, D. L., Swayne, D. E., Stallknecht, D. E., Slemons, R. D., Pedersen, J. C., Senne, D. A., Winker, K., Afonso, C. L. (2007). Phylogenetic Diversity among Low-Virulence Newcastle Disease Viruses from Waterfowl and Shorebirds and Comparison of Genotype Distributions to Those of Poultry-Origin Isolates. J. Virol. 81: 12641-12653 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»