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Journal of Clinical Microbiology, April 2007, p. 1330-1332, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02028-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Colorimetric Phage-Based Assay for Detection of Rifampin-Resistant Mycobacterium tuberculosis

Ruth McNerney,^1* Kim Mallard,¹ Honorathy M. R. Urassa,^1, Eshetu Lemma,² and Helen D. Donoghue³

London School of Hygiene & Tropical Medicine, London, United Kingdom,¹ Infectious and Other Diseases Research Department, Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia,² Centre for Infectious Diseases and International Health, Department of Infection, University College London, London, United Kingdom³

Received 2 October 2006/ Returned for modification 4 December 2006/ Accepted 5 February 2007

Tests based on bacteriophage replication enable rapid screening of Mycobacterium tuberculosis for drug resistance. We describe a novel broth-based colorimetric method for detecting phage replication. When clinical isolates were tested by this novel method, high concordance was observed with both the traditional phage assay and gene mutation analysis for detection of resistance to rifampin.

Published ahead of print on 14 February 2007.

Present address: Ifakara Health Research and Development Centre, P.O. Box 53, Ifakara, Morogoro, Tanzania.