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Journal of Clinical Microbiology, May 2007, p. 1410-1414, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02301-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

High-Resolution Genotyping of Chlamydia trachomatis Strains by Multilocus Sequence Analysis{triangledown}

Markus Klint,1 Hans-Henrik Fuxelius,2 Renée Röstlinger Goldkuhl,1 Hanna Skarin,1 Christian Rutemark,1 Siv G. E. Andersson,2 Kenneth Persson,3 and Björn Herrmann1*

Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden,1 Department of Molecular Evolution, EBC, Uppsala University, Uppsala, Sweden,2 Department of Clinical Microbiology, Malmö University Hospital, Malmö, Sweden3

Received 13 November 2006/ Returned for modification 4 January 2007/ Accepted 15 February 2007

Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequate method is available for analysis of the spread of chlamydial infections in the community. We have developed a multilocus sequence typing (MLST) system based on five target regions and compared it with analysis of ompA, the single gene most extensively used for genotyping. Sequence determination of 16 reference strains, comprising all major serotypes, serotypes A to L3, showed that the number of genetic variants in the five separate target regions ranged from 8 to 16. The genetic variation in 47 clinical C. trachomatis isolates of representative serotypes (14 serotype D, 12 serotype E, 11 serotype G, and 10 serotype K strains) was analyzed; and the MLST system detected 32 variants, whereas 12 variants were detected by using ompA analysis. Specimens of the predominant serotype, serotype E, were differentiated into seven genotypes by MLST but into only two by ompA analysis. The MLST system was applied to C. trachomatis specimens from a population of men who have sex with men and was able to differentiate 10 specimens of one predominant ompA genotype G variant into four distinct MLST variants. To conclude, our MLST system can be used to discriminate C. trachomatis strains and can be applied to high-resolution molecular epidemiology.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden. Phone: 46 18 6113952. Fax: 46 18 559157. E-mail: bjorn.herrmann{at}medsci.uu.se

{triangledown} Published ahead of print on 28 February 2007.

Journal of Clinical Microbiology, May 2007, p. 1410-1414, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02301-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Petrovay, F., Balla, E., Nemeth, I., Gonczol, E. (2009). Genotyping of Chlamydia trachomatis from the endocervical specimens of high-risk women in Hungary. J Med Microbiol 58: 760-764 [Abstract] [Full Text]  
  • Hadad, R, Fredlund, H, Unemo, M (2009). Evaluation of the new COBAS TaqMan CT test v2.0 and impact on the proportion of new variant Chlamydia trachomatis by the introduction of diagnostics detecting new variant C trachomatis in Orebro county, Sweden. Sex. Transm. Infect. 85: 190-193 [Abstract] [Full Text]  
  • Mabey, D. (2008). Commentary on "Chlamydia trachomatis OmpA genotyping as a tool for studying the natural history of genital chlamydial infection". Sex. Transm. Infect. 84: 544-545 [Full Text]  
  • Herrmann, B. (2007). A new genetic variant of Chlamydia trachomatis. Sex. Transm. Infect. 83: 253-254 [Full Text]  


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