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Journal of Clinical Microbiology, May 2007, p. 1415-1419, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02423-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Two Highly Discriminatory Molecular Fingerprinting Assays for Analysis of Multiple Aspergillus fumigatus Isolates from Patients with Invasive Aspergillosis{triangledown}

Hanneke A. de Valk,1 Jacques F. G. M. Meis,1 Ben E. de Pauw,2,3 Peter J. Donnelly,3 and Corné H. W. Klaassen1*

Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands,1 Department of Blood Transfusion and Clinical Immunology,2 Department of Hematology, Academic Medical Center Nijmegen St. Radboud, Nijmegen, The Netherlands3

Received 1 December 2006/ Returned for modification 18 January 2007/ Accepted 8 March 2007

Two highly discriminatory fingerprinting assays, short tandem repeat typing and amplified fragment length polymorphism (AFLP), were compared to determine the genetic relatedness between 55 isolates of Aspergillus fumigatus obtained from 15 different patients suffering from proven invasive aspergillosis. Both techniques showed that interpatient isolates belonged to different genotypes and that intrapatient isolates from deep sites were all of the same genotype. By contrast, multiple genotypes were found among isolates originating from respiratory samples. Both techniques have specific advantages and disadvantages. AFLP is more universally applicable, but short tandem repeat analysis offers better discriminatory power and should be the preferred method for standardizing typing of clinical isolates of Aspergillus fumigatus.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, The Netherlands. Phone: 31-24-3657514. Fax: 31-24-3657516. E-mail: c.klaassen{at}cwz.nl

{triangledown} Published ahead of print on 21 March 2007.

Journal of Clinical Microbiology, May 2007, p. 1415-1419, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02423-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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