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Journal of Clinical Microbiology, May 2007, p. 1469-1477, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.00064-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of the Pathogenic Fungus Aspergillus fumigatus{triangledown}

J. M. Bain,1 A. Tavanti,2 A. D. Davidson,1 M. D. Jacobsen,1 D. Shaw,1 N. A. R. Gow,1 and F. C. Odds1*

Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom,1 Dipartimento di Biologia, Via San Zeno 35-39, Università di Pisa, 56127 Pisa, Italy2

Received 9 January 2007/ Returned for modification 15 February 2007/ Accepted 9 March 2007

A multilocus sequence typing (MLST) scheme was devised for Aspergillus fumigatus. The system involved sequencing seven gene fragments and was applied to a panel of 100 isolates of A. fumigatus from diverse sources. Thirty different sequence types were found among the 100 isolates, and 93% of the isolates differed from the other isolates by only one allele sequence, forming a single clonal cluster as indicated by the eBURST algorithm. The discriminatory power of the MLST method was only 0.93. These results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity. Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination. Mating type data for the 100 isolates showed that 71 isolates were type MAT1-2 and 29 isolates were MAT1-1.

* Corresponding author. Mailing address: Institute of Medical Sciences, Aberdeen AB25 2ZD, United Kingdom. Phone and Fax: 44 1224 555828. E-mail: f.odds{at}abdn.ac.uk

{triangledown} Published ahead of print on 21 March 2007.

Journal of Clinical Microbiology, May 2007, p. 1469-1477, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.00064-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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