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Journal of Clinical Microbiology, May 2007, p. 1611-1613, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02556-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of the AdvanDx VRE EVIGENE Assay for Detection of vanA in Vancomycin-Resistant Staphylococcus aureus{triangledown}

G. E. Fosheim, R. B. Carey, and B. M. Limbago*

Division of Healthcare Quality Promotion, National Center for Preparedness, Detection, and Control of Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS C-16, Atlanta, Georgia 30333

Received 20 December 2006/ Returned for modification 13 February 2007/ Accepted 22 February 2007

AdvanDx VRE EVIGENE, a commercial vanA/vanB DNA hybridization assay to identify vancomycin-resistant enterococci (VRE), was evaluated for the detection of vanA in Staphylococcus aureus. Performance was assessed using S. aureus, VRE, and vancomycin-intermediate and -susceptible isolates. The assay demonstrated 100% sensitivity and specificity when analyzed visually and by optical density.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, MS C16, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-2162. Fax: (404) 639-3822. E-mail: BLimbago{at}cdc.gov

{triangledown} Published ahead of print on 7 March 2007.

Journal of Clinical Microbiology, May 2007, p. 1611-1613, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02556-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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