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Journal of Clinical Microbiology, May 2007, p. 1624-1627, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02316-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Genetic Polymorphisms Influence Plasmodium ovale PCR Detection Accuracy{triangledown}

A. Calderaro,1* G. Piccolo,1 F. Perandin,2 C. Gorrini,1 S. Peruzzi,1 C. Zuelli,1,{dagger} L. Ricci,3 N. Manca,2 G. Dettori,1 C. Chezzi,1 and G. Snounou4

Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Viale A. Gramsci, 14-43100 Parma, Italy,1 Department of Experimental and Applied Medicine, Institute of Microbiology, Spedali Civili, University of Brescia, Piazza Spedali Civili, 1-25123 Brescia, Italy,2 Arcispedale S. Maria Nuova, Centrale Operativa, Viale Risorgimento, 57-42100 Reggio Emilia, Italy,3 Parasitologie Comparée et Modèles Expérimentaux (Unité Scientifique Muséum 0307), Centre National de la Recherche Scientifique Institut Fédératif de Recherche 101, Muséum National d'Histoire Naturelle, CP52, 61 Rue Buffon 75231 Paris Cedex 05, France4

Received 15 November 2006/ Returned for modification 1 January 2007/ Accepted 3 March 2007

Detection of Plasmodium ovale by use of a nested PCR assay with a novel Plasmodium ovale primer set was superior to detection of Plasmodium ovale by real-time PCR assays. Nested PCR was also better at detecting P. malariae. The detection of P. ovale in many patients first admitted >2 months following their return to Italy indicated that P. ovale relapses are common.

* Corresponding author. Mailing address: Faculty of Medicine and Surgery, University of Parma, Department of Pathology and Laboratory Medicine, Section of Microbiology, Viale A. Gramsci, 14-43100 Parma, Italy. Phone: 39 0521 988885. Fax: 39 0521 993620. E-mail: adriana.calderaro{at}unipr.it

{triangledown} Published ahead of print on 14 March 2007.

{dagger} Present address: Department of Laboratory Diagnostic, Unit of Immunohematology and Transfusion, University Hospital of Parma, Viale A. Gramsci, 14-43100 Parma, Italy.

Journal of Clinical Microbiology, May 2007, p. 1624-1627, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02316-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shokoples, S. E., Ndao, M., Kowalewska-Grochowska, K., Yanow, S. K. (2009). Multiplexed Real-Time PCR Assay for Discrimination of Plasmodium Species with Improved Sensitivity for Mixed Infections. J. Clin. Microbiol. 47: 975-980 [Abstract] [Full Text]  


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