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Surveillance for Reassortant Virus by Multiplex Reverse Transcription-PCR Specific for Eight Genomic Segments of Avian Influenza A H5N1 Viruses ^,

Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Bangkok,¹ Faculty of Veterinary Science, Nakhon Pathom,² Faculty of Science, Bangkok,³ Mahidol University, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom,⁴ Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand⁵

Received 19 February 2007/ Returned for modification 23 February 2007/ Accepted 14 March 2007

Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex reverse transcription-PCR assay that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate surveillance for a virus resulting from reassortment between human influenza virus and the H5N1 virus.

Supplemental material for this article may be found at http://jcm.asm.org/.

Published ahead of print on 21 March 2007.