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Journal of Clinical Microbiology, June 2007, p. 1753-1758, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02134-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Genetic and Antigenic Analysis of Invasive Serogroup Y Neisseria meningitidis Isolates Collected from 1999 to 2003 in Canada ^,

Raymond S. W. Tsang,^1* Averil M. Henderson,¹ Marissa L. Cameron,¹ Shaun D. Tyler,² Shari Tyson,² Dennis K. S. Law,¹ Jan Stoltz,¹ and Wendell D. Zollinger³

Laboratory for Vaccine Preventable Bacterial Diseases,¹ DNA Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada,² Department of Bacterial Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland³

Received 17 October 2006/ Returned for modification 8 January 2007/ Accepted 9 April 2007

One hundred forty serogroup Y Neisseria meningitidis isolates recovered from patients with invasive meningococcal disease (IMD) in Canada from 1999 to 2003 were analyzed by genetic and serological methods. Seventy-four isolates (52.9%) belonged to serotype 2c, and most have serosubtype antigen P1.5,2 (37 isolates, 26%) or P1.5 (31 isolates, 22%). Forty-eight isolates (34.3%) belonged to serotype 14 and have serosubtype antigen P1.5,2 (13 isolates, 9%) or P1.5 (7 isolates, 5%) or were nonserosubtypeable (27 isolates, 19%). Thirteen isolates (9.3%) were nonserotypeable. Multilocus sequence typing identified two unrelated clonal populations of serogroup Y meningococci causing invasive disease in Canada: ST-23 and ST-167 clonal complexes. Almost all ST-167-related isolates were typed as 2c:P1.5, while strains of the ST-23 clonal complex were either serotype 14 or 2c but with the serosubtype antigen P1.5,2. In contrast to previous reports that patients with serogroup Y disease are usually older, 26% of the Canadian serogroup Y cases were found in the 10-to-19-year-old age group and another 11% were in the 20-to-39-year-old age group.

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* Corresponding author. Mailing address: Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-6020. Fax: (204) 789-2018. E-mail: Raymond_Tsang{at}phac-aspc.gc.ca

Published ahead of print on 18 April 2007.

Supplemental material for this article may be found at http://jcm.asm.org/.

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Journal of Clinical Microbiology, June 2007, p. 1753-1758, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02134-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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