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Journal of Clinical Microbiology, June 2007, p. 1789-1793, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.01960-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

PCR-Restriction Fragment Length Polymorphism for Rapid, Low-Cost Identification of Isoniazid-Resistant Mycobacterium tuberculosis{triangledown}

Maxine Caws,1,4* Dau Quang Tho,1 Phan Minh Duy,1 Nguyen Thi Ngoc Lan,2 Dai Viet Hoa,2 Mili Estee Torok,1,4 Tran Thi Hong Chau,3 Nguyen Van Vinh Chau,3 Nguyen Tran Chinh,3 and Jeremy Farrar1,4

Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam,1 Pham Ngoc Thach Hospital for Tuberculosis and Lung Diseases, Hung Vuong, District 5, Ho Chi Minh City, Vietnam,2 The Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam,3 Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, Old Road, Headington, Oxford, United Kingdom4

Received 21 September 2006/ Returned for modification 3 February 2007/ Accepted 29 March 2007

PCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-resistant katG_315 mutants. The test had a sensitivity of 80% and a specificity of 100% against conventional phenotypic drug susceptibility testing. The positive and negative predictive values were 1 and 0.86, respectively. None of the discrepant isolates had mutant katG_315 codons by sequencing. The test is cheap (less than $1.50 per test), specific, and suitable for the rapid identification of isoniazid resistance in regions with a high prevalence of katG_315 mutants among isoniazid-resistant M. tuberculosis isolates.

* Corresponding author. Mailing address: Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Quan 5, Ho Chi Minh City, Vietnam. Phone: (84 8) 8384013. Fax: (84 8) 8353904. E-mail: mcaws{at}hotmail.com

{triangledown} Published ahead of print on 11 April 2007.

Journal of Clinical Microbiology, June 2007, p. 1789-1793, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.01960-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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