This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bovers, M.
Right arrow Articles by Boekhout, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bovers, M.
Right arrow Articles by Boekhout, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2007, p. 1874-1883, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00223-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of Genotypically Diverse Cryptococcus neoformans and Cryptococcus gattii Isolates by Luminex xMAP Technology{triangledown}

M. Bovers,1 M. R. Diaz,2 F. Hagen,1 L. Spanjaard,3 B. Duim,4 C. E. Visser,4 H. L. Hoogveld,5 J. Scharringa,6 I. M. Hoepelman,6 J. W. Fell,2 and T. Boekhout1,6*

CBS—Fungal Biodiversity Centre, Utrecht, The Netherlands,1 University of Miami, Rosenstiel School of Marine and Atmospheric Science (RSMAS), Key Biscayne, Florida,2 The Netherlands Reference Laboratory for Bacterial Meningitis (AMC/RIVM), Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands,3 Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands,4 Netherlands Institute of Ecology (NIOO-KNAW), Centre for Limnology, Nieuwersluis, The Netherlands,5 Department of Internal Medicine and Infectious Diseases, University Medical Centre Utrecht, Utrecht, The Netherlands6

Received 29 January 2007/ Returned for modification 5 March 2007/ Accepted 11 April 2007

A Luminex suspension array, which had been developed for identification of Cryptococcus neoformans and Cryptococcus gattii isolates, was tested by genotyping a set of 58 mostly clinical isolates. All genotypes of C. neoformans and C. gattii were included. In addition, cerebrospinal fluid (CSF) obtained from patients with cryptococcal meningitis was used to investigate the feasibility of the technique for identification of the infecting strain. The suspension array correctly identified haploid isolates in all cases. Furthermore, hybrid isolates possessing two alleles of the Luminex probe region could be identified as hybrids. In CSF specimens, the genotype of the cryptococcal strains responsible for infection could be identified after optimization of the PCR conditions. However, further optimization of the DNA extraction protocol is needed to enhance the usability of the method in clinical practice.

* Corresponding author. Mailing address: CBS—Fungal Biodiversity Centre, Uppsalalaan 8, NL-3584CT Utrecht, The Netherlands. Phone: 31(0)30-2122671. Fax: 31(0)30-2512097. E-mail: boekhout{at}cbs.knaw.nl

{triangledown} Published ahead of print on 18 April 2007.

Journal of Clinical Microbiology, June 2007, p. 1874-1883, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00223-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Klein, K. R., Hall, L., Deml, S. M., Rysavy, J. M., Wohlfiel, S. L., Wengenack, N. L. (2009). Identification of Cryptococcus gattii by Use of L-Canavanine Glycine Bromothymol Blue Medium and DNA Sequencing. J. Clin. Microbiol. 47: 3669-3672 [Abstract] [Full Text]  
  • Etienne, K. A., Kano, R., Balajee, S. A. (2009). Development and Validation of a Microsphere-Based Luminex Assay for Rapid Identification of Clinically Relevant Aspergilli. J. Clin. Microbiol. 47: 1096-1100 [Abstract] [Full Text]  
  • Feng, X., Yao, Z., Ren, D., Liao, W. (2008). Simultaneous identification of molecular and mating types within the Cryptococcus species complex by PCR-RFLP analysis. J Med Microbiol 57: 1481-1490 [Abstract] [Full Text]  
  • Gu, A.-D., Mo, H.-Y., Xie, Y.-B., Peng, R.-J., Bei, J.-X., Peng, J., Li, M.-Y., Chen, L.-Z., Feng, Q.-S., Jia, W.-H., Zeng, Y.-X. (2008). Evaluation of a Multianalyte Profiling Assay and an Enzyme-Linked Immunosorbent Assay for Serological Examination of Epstein-Barr Virus-Specific Antibody Responses in Diagnosis of Nasopharyngeal Carcinoma. CVI 15: 1684-1688 [Abstract] [Full Text]  
  • Odds, F. C., Jacobsen, M. D. (2008). Multilocus Sequence Typing of Pathogenic Candida Species. Eukaryot Cell 7: 1075-1084 [Full Text]  
  • Wang, Q.-M., Li, J., Wang, S.-A., Bai, F.-Y. (2008). Rapid Differentiation of Phenotypically Similar Yeast Species by Single-Strand Conformation Polymorphism Analysis of Ribosomal DNA. Appl. Environ. Microbiol. 74: 2604-2611 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»