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Journal of Clinical Microbiology, June 2007, p. 1874-1883, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00223-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of Genotypically Diverse Cryptococcus neoformans and Cryptococcus gattii Isolates by Luminex xMAP Technology

CBS—Fungal Biodiversity Centre, Utrecht, The Netherlands,¹ University of Miami, Rosenstiel School of Marine and Atmospheric Science (RSMAS), Key Biscayne, Florida,² The Netherlands Reference Laboratory for Bacterial Meningitis (AMC/RIVM), Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands,³ Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands,⁴ Netherlands Institute of Ecology (NIOO-KNAW), Centre for Limnology, Nieuwersluis, The Netherlands,⁵ Department of Internal Medicine and Infectious Diseases, University Medical Centre Utrecht, Utrecht, The Netherlands⁶

Received 29 January 2007/ Returned for modification 5 March 2007/ Accepted 11 April 2007

A Luminex suspension array, which had been developed for identification of Cryptococcus neoformans and Cryptococcus gattii isolates, was tested by genotyping a set of 58 mostly clinical isolates. All genotypes of C. neoformans and C. gattii were included. In addition, cerebrospinal fluid (CSF) obtained from patients with cryptococcal meningitis was used to investigate the feasibility of the technique for identification of the infecting strain. The suspension array correctly identified haploid isolates in all cases. Furthermore, hybrid isolates possessing two alleles of the Luminex probe region could be identified as hybrids. In CSF specimens, the genotype of the cryptococcal strains responsible for infection could be identified after optimization of the PCR conditions. However, further optimization of the DNA extraction protocol is needed to enhance the usability of the method in clinical practice.

Published ahead of print on 18 April 2007.

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