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Journal of Clinical Microbiology, June 2007, p. 1965-1968, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00261-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Addition of neuA, the Gene Encoding N-Acylneuraminate Cytidylyl Transferase, Increases the Discriminatory Ability of the Consensus Sequence-Based Scheme for Typing Legionella pneumophila Serogroup 1 Strains{triangledown}

Sandra Ratzow,1 Valeria Gaia,2 Jürgen Herbert Helbig,1 Norman K. Fry,3 and Paul Christian Lück1*

Institut für Medizinische Mikrobiologie und Hygiene, TU Dresden, Dresden, Germany,1 Instituto Cantonale di Microbiologia, Lugano, Switzerland,2 Health Protection Agency, Centre for Infections, Respiratory and Systemic Infection Laboratory, London, United Kingdom3

Received 1 February 2007/ Returned for modification 18 March 2007/ Accepted 22 March 2007

The standard sequence-based method for the typing of Legionella pneumophila serogroup 1 strains was extended by using the gspA and neuA alleles. The use of neuA as a seventh allele for typing significantly increased the index of discrimination calculated for a panel of unrelated strains (from 0.932 to 0.963) and subdivided some known large common complexes (e.g., 1,4,3,1,1,1). This modification to the standard method is proposed as the method of choice in the epidemiological investigation of L. pneumophila infections.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, TU Dresden, Fiedlerstrasse 42, D-01307 Dresden, Germany. Phone: 0049-351-458 65 80. Fax: 0049-351-458 63 10. E-mail: Christian.Lueck{at}mailbox.tu-dresden.de

{triangledown} Published ahead of print on 18 April 2007.

Journal of Clinical Microbiology, June 2007, p. 1965-1968, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00261-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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