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Journal of Clinical Microbiology, June 2007, p. 1972-1973, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02515-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of the Digene Hybrid Capture System Cytomegalovirus (CMV) DNA (Version 2.0), Roche CMV UL54 Analyte-Specific Reagent, and QIAGEN RealArt CMV LightCycler PCR Reagent Tests Using AcroMetrix OptiQuant CMV DNA Quantification Panels and Specimens from Allogeneic-Stem-Cell Transplant Recipients{triangledown}

Kimberly E. Hanson,1,2* L. Barth Reller,1,2 Joanne Kurtzberg,3 Mitchell Horwitz,4 Gwynn Long,4 and Barbara D. Alexander1,2

Division of Infectious Diseases and International Health,1 Division of Cellular Therapy, Department of Medicine,4 Pediatric Blood and Marrow Transplant Program, Department of Pediatrics,3 Clinical Microbiology Laboratory, Department of Pathology, Duke University Medical Center, Durham, North Carolina2

Received 15 December 2006/ Returned for modification 29 January 2007/ Accepted 9 February 2007

The Digene Hybrid Capture system cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests were compared using whole-virus standards and plasma specimens collected from allogeneic-stem-cell transplant recipients. PCR assays showed better speed, sensitivity, and specificity.

* Corresponding author. Mailing address: Duke University Medical Center, Box 3879, Durham, NC 27710. Phone: (919) 668-0789. Fax: (919) 684-8519. E-mail: kim.hanson{at}duke.edu

{triangledown} Published ahead of print on 21 February 2007.

Journal of Clinical Microbiology, June 2007, p. 1972-1973, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02515-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gimeno, C., Solano, C., Latorre, J. C., Hernandez-Boluda, J. C., Clari, M. A., Remigia, M. J., Furio, S., Calabuig, M., Tormo, N., Navarro, D. (2008). Quantification of DNA in Plasma by an Automated Real-Time PCR Assay (Cytomegalovirus PCR Kit) for Surveillance of Active Cytomegalovirus Infection and Guidance of Preemptive Therapy for Allogeneic Hematopoietic Stem Cell Transplant Recipients. J. Clin. Microbiol. 46: 3311-3318 [Abstract] [Full Text]  


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