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Journal of Clinical Microbiology, June 2007, p. 1985-1988, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00159-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Multiplex PCR Assay for Rapid and Accurate Capsular Typing of Group B Streptococci{triangledown}

Claire Poyart,1,2,3* Asmaa Tazi,1,2,3 Hélène Réglier-Poupet,1,2,3 Annick Billoët,1 Nicole Tavares,1 Josette Raymond,1 and Patrick Trieu-Cuot2,4

Service de Bactériologie,1 Centre National de Référence des Streptocoques, Groupe Hospitalier Cochin-Saint Vincent de Paul, Assistance Publique-Hôpitaux de Paris, 27 rue du Faubourg Saint Jacques, 75679 Paris, France,2 Institut Cochin-INSERM U567-UMR CNRS 810, Faculté de Médecine Université Descartes, 22 rue Méchain, 75014 Paris, France,3 Unité de Biologie des Bactéries Pathogènes à Gram-Positif-URA CNRS 2172, Institut Pasteur, 75724 Paris, France4

Received 22 January 2007/ Returned for modification 5 March 2007/ Accepted 12 March 2007

We developed a simple, specific, and sensitive two-multiplex-PCR assay that enabled the detection of all known group B streptococcal (GBS) capsular polysaccharides. This test is well adapted for GBS capsular polysaccharide typing in large-scale epidemiological studies.

* Corresponding author. Mailing address: Service de Bactériologie, Centre National de Référence des Streptocoques, Institut Cochin, INSERM567, Faculté de Médecine Paris Descartes, 27 rue du Faubourg Saint Jacques, 75014 Paris, France. Phone: 33 1 58 41 15 60. Fax: 33 1 58 41 15 48. E-mail: claire.poyart{at}cch.aphp.fr

{triangledown} Published ahead of print on 21 March 2007.

Journal of Clinical Microbiology, June 2007, p. 1985-1988, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00159-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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