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Journal of Clinical Microbiology, June 2007, p. 2048-2050, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00961-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of the Phenotyping Methods ID 32E and VITEK 2 Compact GN with 16S rRNA Gene Sequencing for the Identification of Enterobacter sakazakii{triangledown}

Nadège Fanjat,1 Alexandre Leclercq,2 Han Joosten,3 and Denis Robichon1*

bioMérieux, Research and Development Microbiology, La Balme Les Grottes, France,1 Institut Pasteur, Microbiology Department, 28 rue du Dr Roux, 75724 Paris cedex 15, France,2 Nestle Research Centre, Quality and Safety Department, CH-1000 Lausanne 26, Switzerland3

Received 8 May 2006/ Returned for modification 23 June 2006/ Accepted 30 March 2007

A total of 34 isolates (28 Enterobacter sakazakii and 6 Enterobacteriaceae) from infant formulae, milk powder, and the production environment of milk powder factories were identified using ID 32E and VITEK 2 compact GN systems (bioMérieux, France). The ID 32E version 3.0 and VITEK 2 compact GN version 01.01b correctly identified 100% (28) of the Enterobacter sakazakii isolates tested, whereas the previous software version 2.0 for ID 32E showed only 71.4% correct results. None of the non-E. sakazakii isolates tested were misidentified as E. sakazakii with either of the identification systems used.

* Corresponding author. Mailing address: bioMerieux, Research and Development Microbiology, 3 Route de Port Michaud, 38 390 La Balme Les Grottes, France. Phone: 33 (0)4 74 95 26 22. Fax: 33 (0)4 74 95 26 32. E-mail: denis.robichon{at}eu.biomerieux.com

{triangledown} Published ahead of print on 11 April 2007.

Journal of Clinical Microbiology, June 2007, p. 2048-2050, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00961-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Iversen, C., Lehner, A., Mullane, N., Marugg, J., Fanning, S., Stephan, R., Joosten, H. (2007). Identification of "Cronobacter" spp. (Enterobacter sakazakii). J. Clin. Microbiol. 45: 3814-3816 [Abstract] [Full Text]  


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