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Journal of Clinical Microbiology, June 2007, p. 2058-2060, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02467-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Influence of Disk Preparation on Detection of Metallo-ß-Lactamase-Producing Isolates by the Combined Disk Assay{triangledown}

Soraya S. Andrade,1* Renata C. Picão,2 Eloiza H. Campana,2 Adriana G. Nicoletti,2 Antônio C. C. Pignatari,1 and Ana C. Gales1,2

Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil,1 Laboratório ALERTA, Universidade Federal de São Paulo, São Paulo, Brazil2

Received 8 December 2006/ Returned for modification 22 January 2007/ Accepted 27 March 2007

The combined disk assay has been used for detection of metallo-ß-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many ß-lactam/metallo-ß-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 ß-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.

* Corresponding author. Mailing address: Rua Leandro Dupret, 188, São Paulo, SP 04025-010, Brazil. Phone: 55-11-5081-2819. Fax: 55-11-5571-5180. E-mail: soraya.andrade{at}lemc.com.br

{triangledown} Published ahead of print on 4 April 2007.

Journal of Clinical Microbiology, June 2007, p. 2058-2060, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02467-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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