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Journal of Clinical Microbiology, July 2007, p. 2123-2125, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00691-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Direct Detection of Mycobacterium tuberculosis Rifampin Resistance by a Nitrate Reductase Assay Applied to Sputum Samples in Cotonou, Benin{triangledown}

Dissou Affolabi,1,2 Mathieu Odoun,1 Anandi Martin,2 Juan Carlos Palomino,2 Séverin Anagonou,1 and Françoise Portaels2*

Laboratoire de Référence des Mycobactéries, BP 817 Cotonou Bénin,1 Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp 2000, Belgium2

Received 29 March 2007/ Returned for modification 18 April 2007/ Accepted 24 April 2007

The aim of this study was to evaluate a nitrate reductase assay (NRA) performed on smear-positive sputa for the direct detection of rifampin resistance in Mycobacterium tuberculosis. A total of 213 smear-positive sputa with a positivity score of 1+ or more (>1 acid-fast bacillus per field by fluorescence microscopy) were used in the study. The samples were decontaminated using the modified Petroff method, and portions of the resulting suspension were used to perform the NRA. The NRA results were compared with the reference indirect proportion method for 177 specimens for which comparable results were available. NRA results were obtained at day 10 for 15 specimens (9%), results for 88 specimens (50%) were obtained at day 14, results for 66 specimens (37%) were obtained at day 18, and results for the remaining 8 specimens (4%) were obtained at day 28. Thus, 96% of NRA results were obtained in 18 days. Of the 177 specimens, there was only one discrepancy (susceptible according to the NRA and resistant according to the indirect proportion method). NRA is simple to perform and provides a rapid, accurate, and cost-effective means for the detection of rifampin resistance in M. tuberculosis isolates.

* Corresponding author. Mailing address: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium. Phone: 32-3-247-63-17. Fax: 32-3-247-63-33. E-mail: portaels{at}itg.be

{triangledown} Published ahead of print on 2 May 2007.

Journal of Clinical Microbiology, July 2007, p. 2123-2125, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00691-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Affolabi, D., Odoun, M., Sanoussi, N., Martin, A., Palomino, J. C., Kestens, L., Anagonou, S., Portaels, F. (2008). Rapid and Inexpensive Detection of Multidrug-Resistant Mycobacterium tuberculosis with the Nitrate Reductase Assay Using Liquid Medium and Direct Application to Sputum Samples. J. Clin. Microbiol. 46: 3243-3245 [Abstract] [Full Text]  
  • Affolabi, D., Sanoussi, N., Odoun, M., Martin, A., Koukpemedji, L., Palomino, J. C., Kestens, L., Anagonou, S., Portaels, F. (2008). Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays. J Med Microbiol 57: 1024-1027 [Abstract] [Full Text]  
  • Martin, A., Panaiotov, S., Portaels, F., Hoffner, S., Palomino, J. C., Angeby, K. (2008). The nitrate reductase assay for the rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis. J Antimicrob Chemother 62: 56-64 [Abstract] [Full Text]  


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