JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JCM.00399-07v1
45/7/2180    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Espinel-Ingroff, A.
Right arrow Articles by Walsh, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Espinel-Ingroff, A.
Right arrow Articles by Walsh, T. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2007, p. 2180-2182, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00399-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Quality Control and Reference Guidelines for CLSI Broth Microdilution Method (M38-A Document) for Susceptibility Testing of Anidulafungin against Molds{triangledown}

A. Espinel-Ingroff,1* A. Fothergill,2 M. Ghannoum,3 E. Manavathu,4 L. Ostrosky-Zeichner,5 M. A. Pfaller,6 M. G. Rinaldi,2 W. Schell,7 and T. J. Walsh8

VCU Medical Center, Richmond, Virginia,1 University of Texas Health Science Center, San Antonio, Texas,2 Case Western Reserve University, Cleveland, Ohio,3 Wayne State University, Detroit, Michigan,4 University of Texas-Houston Medical School, Houston, Texas,5 University of Iowa College of Medicine, Iowa City, Iowa,6 Duke University, Durham, North Carolina,7 National Cancer Institute, Bethesda, Maryland8

Received 21 February 2007/ Returned for modification 2 April 2007/ Accepted 24 April 2007

The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and minimum effective concentrations [MECs]) for evaluating anidulafungin against molds. Anidulafungin MICs (≥50% inhibition) and MECs (morphological hyphal changes) were determined for seven Aspergillus isolates (four species) and one isolate each of Fusarium moniliforme, Fusarium solani, and Paecilomyces variotii and for two Scedosporium apiospermum isolates. The inter- and intralaboratory reproducibilities of 10 replicate tests performed in each laboratory on 10 different days for each isolate was 100% at 24 h (MECs, ≤0.015 µg/ml) for six Aspergillus and P. variotii isolates. The reproducibility was 94 to 96.7% at 72 h (MECs, 1 to 8 µg/ml) for S. apiospermum and 96.7 to 97.5% at 48 h (MICs, ≥32 µg/ml) for both Fusarium isolates. Introduction of these identified optimum testing conditions for anidulafungin into future versions of the M38 document is warranted.

* Corresponding author. Mailing address: VCU Medical Center, 1101 E. Marshall Street, Sanger Hall, Room 7-049, P.O. Box 980049, Richmond, VA 23298-0049. Phone: (804) 828-5743. Fax: (804) 828-3097. E-mail: avingrof{at}vcu.edu

{triangledown} Published ahead of print on 2 May 2007.

Journal of Clinical Microbiology, July 2007, p. 2180-2182, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00399-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2007 by the American Society for Microbiology. All rights reserved.