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Journal of Clinical Microbiology, July 2007, p. 2270-2273, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.02604-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of the Colorimetric VITEK 2 Card for Identification of Gram-Negative Nonfermentative Rods: Comparison to 16S rRNA Gene Sequencing{triangledown}

A. Zbinden, E. C. Böttger, P. P. Bosshard, and R. Zbinden*

Institut für Medizinische Mikrobiologie, Universität Zürich, CH-8006 Zürich, Switzerland

Received 29 December 2007/ Returned for modification 5 February 2007/ Accepted 10 May 2007

Ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric VITEK 2 card. The VITEK 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. An algorithm combining the colorimetric VITEK 2 card and 16S rRNA gene sequencing for adequate identification of gram-negative nonfermentative rods was developed. According to this algorithm, any identification by the colorimetric VITEK 2 card other than Achromobacter xylosoxidans, Acinetobacter sp., Burkholderia cepacia complex, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia should be subjected to 16S rRNA gene sequencing when accurate identification of nonfermentative rods is of concern.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie, Universität Zürich, Gloriastrasse 32, 8006 Zürich, Switzerland. Phone: 41 44 634 27 00. Fax: 41 44 634 49 06. E-mail: rzbinden{at}immv.unizh.ch

{triangledown} Published ahead of print on 16 May 2007.

Journal of Clinical Microbiology, July 2007, p. 2270-2273, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.02604-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pincus, D. H., Zbinden, R., Zbinden, A., Bosshard, P. P., Bottger, E. C. (2007). New Vitek 2 Colorimetric GN Card for Identification of Gram-Negative Nonfermentative Bacilli. J. Clin. Microbiol. 45: 4094-4095 [Full Text]  


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