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Journal of Clinical Microbiology, July 2007, p. 2274-2277, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00813-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Ultrasensitive p24 Antigen Assay for Diagnosis of Perinatal Human Immunodeficiency Virus Type 1 Infection{triangledown}

Susan A. Fiscus,1* Jeffrey Wiener,2 Elaine J. Abrams,3 Marc Bulterys,2 Ada Cachafeiro,1 and Richard A. Respess2

University of North Carolina at Chapel Hill, Department of Microbiology and Immunology, Chapel Hill, North Carolina,1 Centers for Disease Control and Prevention, National Center for HIV, STD Viral Hepatitis, and TB Prevention, Atlanta, Georgia,2 Department of Pediatrics, Harlem Hospital Center, Columbia University, New York, New York3

Received 17 April 2007/ Accepted 20 April 2007

We evaluated an ultrasensitive p24 antigen enzyme immunosorbent assay on 802 plasma specimens from 582 infants and children of 0 to 180 days of age. Overall sensitivity and specificity were 91.7% and 98.5%, respectively. After exclusion of infants of less than 7 days of age, the sensitivity and specificity were 93.7% and 98.3%, respectively.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 709 Mary Ellen Jones Building, Chapel Hill, NC 27599-7290. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail: fiscussa{at}med.unc.edu

{triangledown} Published ahead of print on 2 May 2007.

Journal of Clinical Microbiology, July 2007, p. 2274-2277, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00813-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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Copyright © 2007 by the American Society for Microbiology. All rights reserved.