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Journal of Clinical Microbiology, July 2007, p. 2312-2315, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00704-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Contribution of Polymorphisms in ankA, gltA, and groESL in Defining Genetic Variants of Anaplasma phagocytophilum{triangledown} ,{dagger}

Sanjay K. Shukla,1* Vijay Aswani,2 Patrick J. Stockwell,1 and Kurt D. Reed1

Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation,1 Department of Internal Medicine and Pediatrics, Marshfield Clinic, Marshfield, Wisconsin 544492

Received 30 March 2007/ Returned for modification 2 May 2007/ Accepted 10 May 2007

Analysis of several nucleotide polymorphisms in polymorphic genes (ankA, gltA, and groESL) from 16S rRNA gene-based genetic variants of Anaplasma phagocytophilum from dogs in the western United States defined at least two sets of multigene polymorphisms to further characterize these variants. The multigene polymorphism approach holds promise for development of a genotyping scheme for this important pathogen.

* Corresponding author. Mailing address: Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, WI 54449. Phone: (715) 389-5363. Fax: (715) 389-3808. E-mail: shukla.sanjay{at}mcrf.mfldclin.edu

{triangledown} Published ahead of print on 16 May 2007.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, July 2007, p. 2312-2315, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00704-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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