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Journal of Clinical Microbiology, August 2007, p. 2359-2364, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00737-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray ^,

Phenix-Lan Quan,^1, Gustavo Palacios,^1, Omar J. Jabado,¹ Sean Conlan,¹ David L. Hirschberg,² Francisco Pozo,³ Philippa J. M. Jack,⁴ Daniel Cisterna,⁵ Neil Renwick,¹ Jeffrey Hui,¹ Andrew Drysdale,¹ Rachel Amos-Ritchie,⁴ Elsa Baumeister,⁵ Vilma Savy,⁵ Kelly M. Lager,⁶ Jürgen A. Richt,⁶ David B. Boyle,⁴ Adolfo García-Sastre,⁷ Inmaculada Casas,³ Pilar Perez-Breña,³ Thomas Briese,¹ and W. Ian Lipkin^1*

Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, New York, New York,¹ Stanford School of Medicine, Palo Alto, California,² Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain,³ CSIRO Livestock Industries, Australian Animal Health Laboratory, Victoria, Australia,⁴ Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina,⁵ National Animal Disease Center, USDA, Ames, Iowa,⁶ Department of Microbiology and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, New York⁷

Received 4 April 2007/ Returned for modification 15 May 2007/ Accepted 21 May 2007

Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.

Published ahead of print on 6 June 2007.

Supplemental material for this article may be found at http://jcm.asm.org/.

Both authors contributed equally to this work.

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