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Journal of Clinical Microbiology, August 2007, p. 2508-2515, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.02252-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Development of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Yersinia enterocolitica subsp. palearctica and Its Application to Bioserogroup 4/O3 Subtyping{triangledown} ,

Rafal Gierczynski,1* Andrey Golubov,2,{dagger} Heinrich Neubauer,3 Jeannette N. Pham,4 and Alexander Rakin2

Department of Bacteriology, National Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland,1 Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie der Ludwig Maximilians Universität München, Pettenkoferstraße 9a, D-80336, Munich, Germany,2 Institute for Bacteria Identifications and Zoonoses, Friedrich-Loeffler-Institute, Naumburger Str. 96a, 07743 Jena, Germany,3 Department of Microbiology, The Prince of Wales Hospital Randwick, Randwick, NSW 2031, Australia4

Received 3 November 2006/ Returned for modification 4 December 2006/ Accepted 29 May 2007

Yersinia enterocolitica bioserogroup 4/O3 is the predominant causative agent of yersiniosis in Europe and North America. Multiple-locus variable-number tandem-repeat analysis (MLVA) was developed to improve the resolution power of classical genotyping methods. MLVA based on six loci was able to distinguish 76 genotypes among 91 Y. enterocolitica isolates of worldwide origin and 41 genotypes among 51 nonepidemiologically linked bioserogroup 4/O3 isolates, proving that it has a high resolution power. However, only a slight correlation of the MLVA genotypes and the geographic distribution of the isolates was observed. Although MLVA was also capable of distinguishing strains of Y. enterocolitica subsp. palearctica O9 and O5,27, there was only a minor correlation between the MLVA genotypes and serogroups. MLVA may be a helpful tool for epidemiological investigations of Y. enterocolitica subsp. palearctica outbreaks.

* Corresponding author. Mailing address: Department of Bacteriology, National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland. Phone: 48-22-5421244. Fax: 48-22-5421307. E-mail: rgierczynski{at}pzh.gov.pl

{triangledown} Published ahead of print on 6 June 2007.

{dagger} Present address: David Axelrod Institute, Wadsworth Center, NYS Department of Health, 22002 Albany, NY 12201-2002.

Journal of Clinical Microbiology, August 2007, p. 2508-2515, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.02252-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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